|
Status |
Public on Oct 22, 2021 |
Title |
Type-2 diabetic adipocyte Exosomes (T2D) 3 |
Sample type |
RNA |
|
|
Source name |
MCF7 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 cell type: breast cancer treatment: Type-2 diabetic adipocyte Exosomes (T2D) 3
|
Treatment protocol |
MCF7 Cancer cells were treated with ND or T2D adipocytes exosomes for 5 days
|
Growth protocol |
MCF7 cells were cultured in DMEM including 10% FBS and 1% antibiotics for an over night
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolated using Qiagen RNA purification protocol and reagents
|
Label |
SYBR Green
|
Label protocol |
N/A
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
Test
|
Data processing |
Ct values were generated by ABI 7500 Fast System Software
|
|
|
Submission date |
Oct 22, 2021 |
Last update date |
Oct 22, 2021 |
Contact name |
Naser Jafari |
E-mail(s) |
njafari@bu.edu
|
Phone |
8592309730
|
Organization name |
Boston University
|
Department |
Hem Onc
|
Lab |
Denis lab
|
Street address |
72 East Concord
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL30889 |
Series (2) |
GSE186431 |
Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S9] |
GSE186432 |
Adipocyte-derived extracellular vesicles promote breast cancer progression in type 2 diabetes |
|