|
| Status |
Public on Jan 10, 2022 |
| Title |
TGA1_replica1 |
| Sample type |
SRA |
| |
|
| Source name |
induced pluripotent stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Endothelial cells derived from iPSC
disease state: TGA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated through the RNeasy Plus kit (Qiagen)
RNA libraries were prepared for sequencing using standard strand specific total RNA seq Illumina protocols; KAPA Library Quantification Kit KK4835
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
sequences were filtered and trimmed using the cutAdapt tool [http://journal.embnet.org/index.php/embnetjournal/article/view/200]
fastq.gz files were treated with Cutadapt and aligned with Kallisto to obtain the count table for each gene
Differential expression analysis of RNAseq expression profiles was done with the EdgeR package
Supplementary_files_format_and_content: Matrix table with raw RNA counts for every RNA and every sample
|
| |
|
| Submission date |
Oct 29, 2021 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Imelda Ontoria-Oviedo |
| E-mail(s) |
imeldaontoria@gmail.com
|
| Organization name |
Instituto de Investigación Sanitaria La Fe
|
| Department |
Regeneration and Heart transplantation Unit
|
| Street address |
Avda Fernando Abril Martorell 106
|
| City |
Valencia |
| ZIP/Postal code |
46026 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN22766273 |
| SRA |
SRX12834723 |
