|
| Status |
Public on Jan 16, 2011 |
| Title |
Sample050620621-African American-Case |
| Sample type |
RNA |
| |
|
| Source name |
Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
race: African American
disease state: early stage breast cancer
tissue: plasma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications. The quality and quantity of the RNA was evaluated by 260/280 ratio using NanoDrop spectrophotometry (NanoDrop ND-1000 Technologies Inc.) and Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Two hundred ng of total RNA from each sample were labeled and hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102–1024; Illumina), according to the manufacturers recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| |
|
| Hybridization protocol |
Two hundred ng of total RNA from each sample were labeled and hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102–1024; Illumina), according to the manufacturers recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
50620621
|
| Data processing |
The data were normalised using quantile normalisation with lumi package in R
|
| |
|
| Submission date |
Jul 16, 2010 |
| Last update date |
Jan 16, 2011 |
| Contact name |
hua zhao |
| E-mail(s) |
Hua.Zhao@RoswellPark.org
|
| Organization name |
Roswell Park Cancer Institute
|
| Street address |
Elm and Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE22981 |
Circulating miRNAs as biomarkers and potential functional mediators of early stage breast cancer |
|
