|
| Status |
Public on Dec 01, 2010 |
| Title |
hTERT immortalized cells, dominant negative peptide for p53 vector , passage 80 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hTERT immortalized cells, dominant negative peptide for p53 vector , passage 80
|
| Treatment protocol |
EP156T immortalized prostate cell cultures were grown in MCDB-153+ media under standard tissue culture conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were trypsinized and pelleted after 3 days at logarithmic growth stage
|
| Label |
biotin
|
| Label protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
samples were taken at 33 points along the cultivation process and hybridized with the GeneChip Human Genome U133A 2.0 Array. The microarrays were processed using RMA (Irizarry et al. 2003) and normalized.
|
| |
|
| Submission date |
Jul 20, 2010 |
| Last update date |
Jul 21, 2010 |
| Contact name |
Yuval Tabach |
| E-mail(s) |
yuval.tabach@weizmann.ac.il
|
| Phone |
972-8-9344985
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Molecular Cell Biology
|
| Street address |
Hativat Givaty 30
|
| City |
Raanana |
| ZIP/Postal code |
43338 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE23038 |
Normal prostate cells were immortalized and cultured for 650 days till several transformation hallmarks were observed |
|
