|
| Status |
Public on Jun 06, 2011 |
| Title |
Mantle_Stage4/Stage3_rep3 [male] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mantle tissue, individual sampled on March 2008, Bizerta Lagoon, Tunisia, stage 4 of male gonad development
|
| Organism |
Mytilus galloprovincialis |
| Characteristics |
gender: male
tissue: Mantle
date: March-08
geographical location: Bizerta Lagoon, Tunisia
developmental stage: stage 4 of male gonad development
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from the mantle of a single individual mussel using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
15 µg of total RNA were primed with 0.5 µg oligodT(19)VN primer at 70°C for 10 min, then reversed transcribed at 42°C for 2 h in the presence of 400 U ReverseAid MuLV H minus RTase (Fermentas), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labeled dCTP or Cy5-label dCTP
|
| |
|
| Channel 2 |
| Source name |
Mantle tissue, individual sampled on February 2008, Bizerta Lagoon, Tunisia, stage 3 of male gonad development
|
| Organism |
Mytilus galloprovincialis |
| Characteristics |
gender: male
tissue: Mantle
date: Feb-08
geographical location: Bizerta Lagoon, Tunisia
developmental stage: stage 3 of male gonad development
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from the mantle of a single individual mussel using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
15 µg of total RNA were primed with 0.5 µg oligodT(19)VN primer at 70°C for 10 min, then reversed transcribed at 42°C for 2 h in the presence of 400 U ReverseAid MuLV H minus RTase (Fermentas), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labeled dCTP or Cy5-label dCTP
|
| |
|
| |
| Hybridization protocol |
43°C, 16/20 h, hybridization buffer Northern Max (Ambion). Pre-hybridization for 1h in same conditions.
|
| Scan protocol |
Scanned on Chip Reader (Bio-rad Laboratories) at 5 µm resolution
|
| Description |
loop design
|
| Data processing |
Offset background subtracted, loess normalized. Data obtained from log2 of processed Ch1 signal / processed Ch2 signal. LIMMA software was used
|
| |
|
| Submission date |
Jul 21, 2010 |
| Last update date |
Jun 06, 2011 |
| Contact name |
Francesco Dondero |
| E-mail(s) |
francesco.dondero@mfn.unipmn.it
|
| Organization name |
University of Piemonte Orientale "Amedeo Avogadro"
|
| Department |
Department of Environmental and Life Sciences
|
| Lab |
Molecular Ecotoxicology
|
| Street address |
Via Michel 11
|
| City |
Alessandria |
| ZIP/Postal code |
15121 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10269 |
| Series (2) |
| GSE23050 |
Mytilus galloprovincialis: development of male gonads |
| GSE23052 |
Mytilus galloprovincialis: temporal expression analysis of digestive gland and mantle |
|
