|
Status |
Public on Dec 12, 2021 |
Title |
WT_preGC_3 |
Sample type |
SRA |
|
|
Source name |
Wild-type Pre-GC B cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: Spleen genotype: Mb1cre(WT/WT) Tbk1(flox/flox) cell type: Germinal center precursor B cells (Pre-GC) agent: Plasmodium yoelii time point: Day 9
|
Growth protocol |
Mb1cre/wt Tbk1f/f mice and Mb1wt/wt Tbk1f/f mice on a C57BL/6 background were infected with Plasmodium yoelii Non-Lethal parasites at 1x10 infected erythrocytes. On day 9, splenic Pre-GC B cells (B220+ IgD- CD38+ GL7+) were enriched by MACS separation and sorted by FACS for RNA preparation. Total RNA was purified from 1 x 10^5 sorted cells.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen) DNA library was constructed using TruSeq Stranded mRNA Sample Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
TopHat version 2.0.13 Bowtie2 version 2.2.3 SAMtools version 0.1.19 Cufflinks version 2.2.1 Genome_build: mm10 Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for each sample
|
|
|
Submission date |
Nov 12, 2021 |
Last update date |
Dec 12, 2021 |
Contact name |
Michelle Sue Jann Lee |
E-mail(s) |
msj-lee@ims.u-tokyo.ac.jp
|
Organization name |
The University of Tokyo
|
Department |
Institute of Medical Science
|
Street address |
Minato-ku, Shirokanedai 4-6-1, Building 1, 3F, Room 333
|
City |
Tokyo |
State/province |
Tokyo |
ZIP/Postal code |
1088639 |
Country |
Japan |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE188659 |
B cell intrinsic TBK1 is essential for germinal center formation during infection and vaccination |
|
Relations |
BioSample |
SAMN23078424 |
SRA |
SRX13117678 |