|
| Status |
Public on Mar 31, 2022 |
| Title |
D1 |
| Sample type |
SRA |
| |
|
| Source name |
Candida albicans
|
| Organism |
Candida albicans |
| Characteristics |
genotype: WT
treatment: DMSO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed by Mini-beadbeater and total RNA was harvested using GeneJET RNA Purification Kit (Thermo Scientific #K0731, #K0732)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The library products were sequenced using an Illumina NovaSeq platform.
The clean reads were aligned to the genome of C. albicans SC5314 using HiSat v2.0.5 with default parameters.
Relative gene expression levels were calculated using the FPKM (Fragments Per kb per Million reads) method. Differentially expressed genes were analyzed with the DESeq2 R package.
Genome_build: Candida albicans SC5314 genome (ASM18296v1)
Supplementary_files_format_and_content: text files containing FPKM values for each Sample
|
| |
|
| Submission date |
Nov 15, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
LI TAO |
| E-mail(s) |
taoli@fudan.edu.cn
|
| Phone |
+862131246551
|
| Organization name |
Fudan University
|
| Street address |
Yangpu district Songhu Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
| |
|
| Platform ID |
GPL28323 |
| Series (1) |
| GSE188886 |
Streptococcus mutans suppresses filamentous growth of Candida albicans through secreting mutanocyclin, an unacylated tetramic acid |
|
| Relations |
| BioSample |
SAMN23167862 |
| SRA |
SRX13143528 |
