|
| Status |
Public on Nov 18, 2021 |
| Title |
S_3 |
| Sample type |
SRA |
| |
|
| Source name |
Fusarium graminearum-infected wheat grains
|
| Organisms |
Triticum aestivum; Fusarium graminearum |
| Characteristics |
tissue: wheat grains
infection status: infected with Fusarium graminearum
cultivar: Sumai 3
|
| Treatment protocol |
One portion for every 25 g of Sumai 3, Zhongmai 66B and Annong 0711 wheat grains was plated into a 100 mL sterile conical flask, and the initial aw of the wheat kernel was 0.572, which was confirmed by using a Novasina Labmaster-Neo water activity meter (Novasina, Inc., WA, Swit). Then, sterile distilled water was added to rehydrate to aw: 0.99. The incubation process was carried out in 20 ℃ and humidity (50%) incubator.
|
| Growth protocol |
Two 5 mm-diameter colonies taken from the edge of F. graminearum strains that were cultivated on PDA medium at 25 ℃ for 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAiso Plus Kits (Takara, China) according to the manufacturer’s recommendation. To eliminate genomic DNA contamination, the DNase I (TaKara, China) was used.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
two paired-end sequencing
|
| Data processing |
Illumina HiSeq X Ten (2×150bp) sequencer with two paired-end was used for sequencing
Sequenced reads were trimmed for adaptor sequence, mapped with reference genome Triticum aestivum (Taxonomy ID 4565) and Fusarium graminearum (Taxonomy ID 5518)
Software RSEM was used to quantitatively analyze gene expression levels respectively
Genome_build: Triticum aestivum (Taxonomy ID 4565) and Fusarium graminearum (Taxonomy ID 5518)
Supplementary_files_format_and_content: tab-delimited text files include TPM values for every gene and every sample
|
| |
|
| Submission date |
Nov 16, 2021 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Kailin Li |
| E-mail(s) |
likailin2018@sibs.ac.cn
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institute of Nutrtion and Health
|
| Lab |
CAS Key Laboratory of Nutrtion, Metabolism and Food Safety
|
| Street address |
Tian Ping
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL30964 |
| Series (1) |
| GSE188959 |
Transcriptome of different cultivars of Fusarium graminearum-infected wheat grains |
|
| Relations |
| BioSample |
SAMN23198531 |
| SRA |
SRX13150008 |
