|
| Status |
Public on Jul 26, 2012 |
| Title |
Soybean_PP1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
palisade mesophyll parenchyma cells
|
| Organism |
Glycine max |
| Characteristics |
cell type: palisade mesophyll chlorenchyma
|
| Treatment protocol |
None, untreated plants were used.
|
| Growth protocol |
Plants were grown in growth chambers in 4-L pots in growth chambers, with 16 hour day-lengths and 8 hour night periods. The daytime temperature was set to 25 oC. The night time temperature was 18oC. The minimum light intensity was 360-400 umol m2/s.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Soybean leaves were fixed with 5:1 ethanol: acetic acid, embedded in low-temp paraffin, and sectioned before used for laser microdissection. RNA was extracted from the laser-microdissected cells using Qiagen Micro Rneasy kit.
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix one cycle labeling and fragmentation protocol
|
| |
|
| Hybridization protocol |
15ug biotinylated cRNA hybridized for 16 hr at 45C in Affymetrix 640 hybridization oven at 50rpm
|
| Scan protocol |
Standard Affymetrix Scan on Affymetrix 7G scanner
|
| Description |
Gene expression within paraveinal mesophyll cells of mature soybean leaves.
|
| Data processing |
The data were analyzed using GeneChip Operating Software with affymetrix default settings utilizing global scaling for normalization and a target intensity value of 125.
|
| |
|
| Submission date |
Jul 23, 2010 |
| Last update date |
Jul 26, 2012 |
| Contact name |
Glenn W Turner |
| E-mail(s) |
gturner@wsu.edu
|
| Phone |
509-335-3863
|
| Fax |
509-335-7643
|
| Organization name |
Washington State University
|
| Department |
Institute of Biological Chemistry
|
| Lab |
Lange Laboratory
|
| Street address |
PO Box 646340, Washington State University
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164-6340 |
| Country |
USA |
| |
|
| Platform ID |
GPL4592 |
| Series (1) |
| GSE23128 |
Comparison of RNA expression in paravienal mesophyll (PVM) and palisade parenchyma (PP) cells. |
|
