|
| Status |
Public on Mar 01, 2011 |
| Title |
Disc Tissue 7 |
| Sample type |
RNA |
| |
|
| Source name |
Disc Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue_grade: III
lcm_or_homogenization: LCM
tissue_source: CHTN specimen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolation via 1.) Cells were collected via laser capture mircodissection (LCM) and total RNA was isolated according to the Paradise reagent system 2.) tissue was homongenized and RNA isolated using TRIzol reagent
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned once via the Affymetrix 3000G scanner.
|
| Data processing |
The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
|
| |
|
| Submission date |
Jul 23, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Helen Gruber |
| E-mail(s) |
Helen.Gruber@carolinashealthcare.org
|
| Organization name |
Carolinas HealthCare System
|
| Department |
Orthopaedic Surgery
|
| Lab |
Orthopaedic Research
|
| Street address |
1542 Graden Terrace
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE23130 |
Gene Array Data from Human Annulus Disc Tissue |
|
