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Sample GSM571 Query DataSets for GSM571
Status Public on Oct 03, 2001
Title HRPE1_SAGE
Sample type SAGE
Anchor NlaIII
Tag Count 53666
Tag Length 10
 
Source name human retinal pigment epithelium (RPE)
Organism Homo sapiens
Extracted molecule total RNA
 
Description Profile of the genes expressed in the human peripheral retina, macula, and retinal pigment epithelium determined through serial analysis of gene expression (SAGE).

A normal retinal pigment epithelium (RPE) from an 88-year-old female.
Patient had periocular malignancies that required exenteration of a normal eye as part of the clinically indicated surgical therapy. The eye was placed in saline after it was removed from the patient and the RPE from the posterior pole and the periphery was harvested by gentle scraping with a Pasteur pipette. Fragments of RPE were collected by aspiration in sterile 0.9% saline. Tissue fragments were collected and frozen within 45 minutes of the surgical removal to ensure minimal degradation of mRNA molecules. The RPE fragments were from the posterior pole and the periphery.
 
Submission date Sep 19, 2001
Last update date Nov 19, 2008
Contact name Dror Sharon
E-mail(s) dror_sharon@meei.harvard.edu
Phone 617-573-4347
Fax 617-573-3168
URL http://eyegene.meei.harvard.edu/
Organization name Massachusetts Eye and Ear
Department
Lab Ocular Molecular Genetics Laboratory
Street address 243 Charles St.
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL4
Series (1)
GSE10 eye-SAGE

Data table header descriptions
TAG Ten base SAGE tag,
COUNT number of copies
TPM tags per million

Data table
TAG COUNT TPM
GTGGCTCACA 1350 25155.59
TCCCCGTACA 592 11031.19
TGGTTGCTGG 457 8515.63
GACTGACCCT 451 8403.83
TCCCTATTAA 335 6242.31
ACTTTTTCAA 221 4118.06
TAACCAAGAG 156 2906.87
TCTCCATACC 139 2590.09
TTCATACACC 120 2236.05
GAAATACAGT 97 1807.48
ACAAAACCAA 80 1490.70
CACCTAATTG 80 1490.70
CTAAGACTTC 76 1416.17
AGCCCTACAA 75 1397.53
CCCATCGTCC 68 1267.10
ATAACACATA 65 1211.20
GCTCCAGCTG 62 1155.29
GGGTTGGGGA 62 1155.29
TGATTTCACT 62 1155.29
ATACTGACAC 59 1099.39

Total number of rows: 38358

Table truncated, full table size 712 Kbytes.




Supplementary data files not provided

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