|
| Status |
Public on Aug 31, 2010 |
| Title |
Female MI (4218331017_B) |
| Sample type |
RNA |
| |
|
| Source name |
left ventricular samples (infarct excluded)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 12-15 weeks
tissue: left ventricle
gender: Female
stress: MI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RNeasy Fibrous Tissue Mini Kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation followed by log2 transform with GeneSpring GX11. P/A flag was based on a P>0.95 cut off. Only genes with at least one P flag were used in the analysis
|
| |
|
| Submission date |
Jul 28, 2010 |
| Last update date |
Aug 31, 2010 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE23294 |
An Association Between Gene Expression and Better Survival in Female Mice Following Myocardial Infarction |
|
