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Sample GSM572458 Query DataSets for GSM572458
Status Public on Nov 16, 2010
Title 15.5M rep2
Sample type RNA
 
Source name PGC E15.5M
Organism Mus musculus
Characteristics gender: male
age: embryonic day 15.5
cell type: primordial germ cells
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Hybridized for 18 hrs onto MouseRef-8 v2 gene expression BeadChips (Illumina) following the manufacturer's instructions.
Scan protocol Chips were scanned using the iScan reader and accompanying software.
Description Replicate 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was performed using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jul 29, 2010
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE23322 Identification of genes specific to mouse primordial germ cells through dynamic global gene expression

Data table header descriptions
ID_REF
VALUE Quantile-normalized signal

Data table
ID_REF VALUE
ILMN_1212607 2.208198
ILMN_1212612 2.361769
ILMN_1212619 3.284608
ILMN_1212628 1.419775
ILMN_1212632 6.105358
ILMN_1212636 9.850076
ILMN_1212637 7.024607
ILMN_1212645 5.377961
ILMN_1212648 8.965469
ILMN_1212653 2.827067
ILMN_1212672 7.459684
ILMN_1212682 3.214341
ILMN_1212683 1.878538
ILMN_1212685 1.923193
ILMN_1212692 7.576945
ILMN_1212693 6.706723
ILMN_1212695 2.554423
ILMN_1212698 2.282828
ILMN_1212702 10.676469
ILMN_1212703 2.218856

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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