|
| Status |
Public on Dec 31, 2010 |
| Title |
chronic GvHD |
| Sample type |
RNA |
| |
|
| Source name |
blood from allogeneic stem cell transplantation (SCT) patient
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: chronic GvHD
cell type: CD4+CD25highCD127low/- Tregs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy, Qiagen
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000.
|
| Description |
chronic GvHD
MHH0899
|
| Data processing |
RMA summarization using GeneSpring GX11 (Agilent Technology)
|
| |
|
| Submission date |
Jul 29, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23332 |
Molecular plasticity of regulatory T cells in allogeneic stem cell transplantation |
|
| Relations |
| Reanalyzed by |
GSE86362 |
