|
Status |
Public on Jul 31, 2010 |
Title |
Liver_normoglycima_no.5 |
Sample type |
RNA |
|
|
Source name |
Liver, fasting
|
Organism |
Homo sapiens |
Characteristics |
tissue: liver gender: male disease status: normal glucose tolerance
|
Treatment protocol |
Patients with type 2 diabetes were treated with diet therapy alone or insulin; no patients were receiving any oral hypoglycaemic agent. Participants receiving statins, ACE inhibitors or angiotensin II receptor blockers were excluded.
|
Growth protocol |
Hepatic tissues were obtained with percutaneous needle liver biopsy, and immediately frozen in liquid nitrogen and stored at −80°C until use.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with a kit (Micro RNA Isolation Kit; Stratagene, La Jolla, CA, USA), purified with RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany).
|
Label |
biotin
|
Label protocol |
A total of 100 ng of total RNA was labelled according to the small sample labelling protocol recommended by the manufacturer.
|
|
|
Hybridization protocol |
Ten micrograms of labelled and fragmented cRNA was hybridised onto a Human Genome U133 Plus 2.0 Array (Affymetrix) for 16 h at 45°C.
|
Scan protocol |
Chips were scanned by using GeneChip Scanner3000, Affymetrix.
|
Description |
Gene expression data from human liver.
|
Data processing |
A scanned image was quantified using GCOS version 1.1.1 (GeneChip Operating Software; Affymetrix) with the default parameters for the statistical algorithm and all probe sets scaled to a target intensity of 100.
|
|
|
Submission date |
Jul 30, 2010 |
Last update date |
Oct 30, 2013 |
Contact name |
Hirofumi Misu |
E-mail(s) |
hmisu@m-kanazawa.jp
|
Phone |
+81-76-265-2234
|
Organization name |
Kanazawa University
|
Street address |
13-1 Takaramachi
|
City |
Kanazawa |
ZIP/Postal code |
920-8641 |
Country |
Japan |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE23343 |
Expression data from human liver with or without type 2 diabetes |
|
Relations |
Reanalyzed by |
GSE51798 |