MCF7 cells were treated with DMSO and 10 μM PCW-1001 for 24 h
Growth protocol
MCF7 cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Corning, NY, USA) supplemented with 10% fetal bovine serum (Corning) and 1% penicillin/streptomycin (GenDEPOT, USA) or 1% ZellShieldTM (Minerva Biolabs, Berlin, Germany). The cells maintained under standard conditions, at humidified atmosphere of 5% CO2 at 37°C.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted by standard protocol
Label
n/a
Label protocol
n/a
Hybridization protocol
n/a
Scan protocol
n/a
Data processing
Quantitative changes in mRNA levels were analyzed and clustered using the nSolver software (NanoString Technologies).
Submission date
Dec 14, 2021
Last update date
Dec 15, 2021
Contact name
Minsung Kang
Organization name
Korea institute of radiological and medical sciences
