|
Status |
Public on Aug 02, 2012 |
Title |
small RNAs from zygotes_SL256 |
Sample type |
SRA |
|
|
Source name |
zygotes 6h
|
Organism |
Chlamydomonas reinhardtii |
Characteristics |
strain: R3+/NO- 3prime adaptor: TCGTATGCCGTCTTCTGCTTGT
|
Treatment protocol |
Small RNAs were isolated from different strains of Chlamydomonas and from cells at different phases of the Chlamydomonas sexual cycle. Genomic DNA was isolated from vegetative cells.
|
Growth protocol |
Chlamydomonas cells were grown in liquid culture.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Trizol (Invitrogen) and small RNA isolated from the total RNA fraction using mirVana (Ambion), adapter sequences appropriate Illumina sequencing were ligated.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
Sequence reads were obtained using the Illumina Genome Analyzer Pipeline. The 3 prime adaptor sequence was removed from the small RNA reads and the reads were aligned to the Chlamydomonas genome, assembly 4. Only reads with 100% match to the genome were retained.
|
|
|
Submission date |
Aug 02, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Krys Kelly |
Organization name |
University of Cambridge
|
Department |
Plant Sciences
|
Lab |
Baulcombe Group
|
Street address |
Downing Street
|
City |
Cambridge |
ZIP/Postal code |
CB2 3EA |
Country |
United Kingdom |
|
|
Platform ID |
GPL9152 |
Series (1) |
GSE23379 |
Small RNA profiling of strains and lifecycle of Chlamydomonas reinhardtii |
|
Relations |
SRA |
SRX025312 |
BioSample |
SAMN00027530 |