|
| Status |
Public on Dec 31, 2023 |
| Title |
AO_24h_2 |
| Sample type |
SRA |
| |
|
| Source name |
Arthrobotrys oligospora and nematode
|
| Organism |
Orbilia oligospora |
| Characteristics |
cell type: Fungal hyphae
time point: 24h
|
| Treatment protocol |
Mycelium was collected and washed three times with 0.1 Mphosphate buffer saline (PBS) (pH 6.8-7.2).
|
| Growth protocol |
The WT and ΔAoswi6 mutant strains were cultured in PD shaking at 28 °C, 180 rpm for 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mycelium was divided and induced with about 600 nematodes for 0, 12, 24, and 36 h, respectively.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
The data from high-throughput sequencing were analyzed using the OmicShare tools, a free online platform for data analysis (www.majorbio.com).
Genome_build: https://www.ncbi.nlm.nih.gov/assembly/GCF_000225545.1
Supplementary_files_format_and_content: Expression analysis table include TPM values for each sample
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Dec 22, 2021 |
| Last update date |
Dec 31, 2023 |
| Contact name |
xie mei hua |
| E-mail(s) |
xiemeihua@cxtc.edu.edu.cn
|
| Phone |
13987080871
|
| Organization name |
Yunnan university
|
| Street address |
121 street
|
| City |
Yunnan Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
675000 |
| Country |
China |
| |
|
| Platform ID |
GPL31134 |
| Series (1) |
| GSE192487 |
Transcriptome analysis of Aoswi6 in nematode-trapping fungus Arthrobotrys oligospora |
|
| Relations |
| BioSample |
SAMN23806549 |
| SRA |
SRX13363566 |
