|
| Status |
Public on Aug 09, 2010 |
| Title |
WT, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wildtype mouse adult fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
tissue: adult primary fibroblasts
strain: C57bl6X129S3/J
|
| Treatment protocol |
Primary adult mouse fibroblasts were cultured from the initial derivation in culture as described above and subjected to three passages at confluency, at a passage ratio of 1:3 according to the 3T3 protocol (Todaro and Green, 1963), beginning one passage after derivation.
|
| Growth protocol |
Primary adult mouse fibroblasts in 10% DMEM medium with standard antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction according to manufacturer's specifications
|
| Label |
biotin
|
| Label protocol |
Affymetrix One Cycle Target Labeling kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450/250.
|
| Scan protocol |
Affymetrix Gene Chip Scanner
|
| Description |
primary cells in culture
|
| Data processing |
BRB-Array Tools version 3.7 (Biometrics Research Branch, NCI, publicly available)
|
| |
|
| Submission date |
Aug 06, 2010 |
| Last update date |
Aug 06, 2010 |
| Contact name |
Lidia Hernandez |
| E-mail(s) |
hernandli@mail.nih.gov
|
| Organization name |
National Cancer Institute
|
| Department |
CCR/MOB
|
| Lab |
Translational Genomics Section
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23495 |
Lmnadelta9 mouse gene expression study |
|
