GEO help: Mouse over screen elements for information.
Status
Public on Oct 11, 2001
Title
BM_CD34-1b
Sample type
RNA
Source name
bone marrow flow-sorted CD34+ cells (>95% purity)
Organism
Homo sapiens
Extracted molecule
total RNA
Description
Comparison of gene expression in CD34+ cells from bone marrow and G-CSF mobilized peripheral blood by high density oligonucleotide array analysis. This sample represents a separate biotinylated cRNA preparation from the cells described in sample BM_CD34-1a (GSM575), hybridized to a different chip. Sample hybridization parameters: Background Avg: 469.14, Stdev: 13.94, Max: 490.4, Min: 449.5 Corner+ Avg:1378, Count:32 Corner- Avg:45744, Count:32 BF= SDT=94.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=17.08 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=94.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 TGT=1000 NF=1.000000 SF=1.383271 SFGene=All
Submission date
Sep 26, 2001
Last update date
May 27, 2005
Contact name
Lynn Graf
E-mail(s)
lgraf@fhcrc.org
Phone
206-667-4545
Fax
206-667-5978
URL
http://none
Organization name
Fred Hutchinson Cancer Research Center
Department
Clinical Research Division
Lab
Torok-Storb
Street address
1100 Fairview Ave. N. D1-100
City
Seattle
State/province
WA
ZIP/Postal code
98109
Country
USA
Platform ID
GPL80
Series (1)
Data table header descriptions
ID_REF
VALUE
AverageDifference hybridization intensity
POSITIVE
number of pairs with positive signal
NEGATIVE
number of pairs with negative signal
PAIRS
number of PerfectMatch and MisMatch oligo pairs specifying sequence
PAIRS_USED
number of pairs used for analysis
PAIRS_IN_AVG
trimmed probe pair set
POS_FRACTION
#positive probe pairs/#of probe pairs used
LOG_AVG
log average ratio (see Affymetrix literature for formula)
POS/NEG
ratio of positive to negative pairs
ABS_CALL
absolute call of Presence or Absence of gene transcript in sample
Data table
ID_REF
VALUE
POSITIVE
NEGATIVE
PAIRS
PAIRS_USED
PAIRS_IN_AVG
POS_FRACTION
LOG_AVG
POS/NEG
ABS_CALL
AFFX-BioB-5_at
22.7
6
2
20
20
18
0.3
0.66
3
A
AFFX-BioB-M_at
83.1
5
1
20
20
18
0.25
0.48
5
A
AFFX-BioB-3_at
391
13
2
20
20
18
0.65
2.47
6.5
P
AFFX-BioC-5_at
475.6
14
0
20
20
19
0.7
3.58
Undef
P
AFFX-BioC-3_at
20.8
5
5
20
20
18
0.25
0
1
A
AFFX-BioDn-5_at
530.3
13
0
20
20
18
0.65
3.13
Undef
P
AFFX-BioDn-3_at
-426.4
7
7
20
20
20
0.35
0.24
1
A
AFFX-CreX-5_at
936.4
17
0
20
20
19
0.85
4.16
Undef
P
AFFX-CreX-3_at
520.3
11
1
20
20
17
0.55
3
11
P
AFFX-BioB-5_st
2714.2
13
1
20
20
19
0.65
3.43
13
P
AFFX-BioB-M_st
133.6
9
3
20
20
17
0.45
0.98
3
M
AFFX-BioB-3_st
898.6
11
1
20
20
19
0.55
1.76
11
P
AFFX-BioC-5_st
-590.2
4
7
20
20
18
0.2
-0.58
0.6
A
AFFX-BioC-3_st
-580.1
8
3
20
20
19
0.4
0.9
2.7
A
AFFX-BioDn-5_st
947.2
14
0
20
20
18
0.7
3.1
Undef
P
AFFX-BioDn-3_st
552.1
11
3
20
20
18
0.55
1.03
3.7
M
AFFX-CreX-5_st
-7.9
6
8
20
20
18
0.3
-0.07
0.8
A
AFFX-CreX-3_st
-310.3
6
7
20
20
18
0.3
-0.16
0.9
A
hum_alu_at
37.7
10
5
20
20
19
0.5
1.46
2
A
AFFX-DapX-5_at
-43.1
9
4
20
20
19
0.45
1.18
2.3
A
Total number of rows: 7129 Table truncated, full table size 320 Kbytes .Supplementary file
Size
Download
File type/resource
GSM576.cel.gz
2.2 Mb
(ftp) (http)
CEL