|
| Status |
Public on Jan 03, 2022 |
| Title |
cervix_local_recurrence_5 |
| Sample type |
RNA |
| |
|
| Source name |
cervix
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cervix
stage at last follow-up: Death
age: 65
surgical approach: Open
histological subtype: squamous
lvsi: yes
tnm-stage: 1b1
hpv: 16
adjuvant therapy: Follow up
time to recurrence: 11
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy® FFPE Kit (Qiagen, Hilden, Q15 Germany) according to the manufacturer’s directions. RNA concentrations and quality were measured using the Agilent 2100 BioAnalyzer (Santa Clara, CA, USA). To correct for degradation of the RNA, the percentage of fragments of 300-4000 nucleotides was used to calculate the corrected concentrations. For each sample, 300 ng of total RNA, with a maximum of 7 mL (>42.8 ng/mL), was used.
|
| Label |
n.a.
|
| Label protocol |
n.a.
|
| |
|
| Hybridization protocol |
Hybridization was performed at 65°C for 17 hours using a SimpliAmp Thermal Cycler (Applied Biosystems, Foster City, CA, USA).
|
| Scan protocol |
The nCounter FLEX platform was used and genes were counted by scanning 490 Fields-of-view (FOV).
|
| Data processing |
Data analysis was performed using the advanced analysis module (version 2.0) of nSolver software (version 4.0, NanoString Technology).
|
| |
|
| Submission date |
Jan 02, 2022 |
| Last update date |
Jan 03, 2022 |
| Contact name |
Willem de Koning |
| E-mail(s) |
w.dekoning.1@erasmusmc.nl
|
| Organization name |
Erasmus University Medical Center
|
| Street address |
Doctor Molewaterplein 40
|
| City |
Rotterdam |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
3015 GD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL27956 |
| Series (1) |
| GSE192897 |
Identifying molecular changes in early cervical cancer samples of patients that developed metastasis |
|
