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Status |
Public on Sep 21, 2010 |
Title |
MEP_induced_rep1 |
Sample type |
RNA |
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Source name |
MEP cells isolated from E13.5 palatal shelves, induced
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Organism |
Mus musculus |
Characteristics |
strain: CBA;C57BL6 N2 developmental stage: embryo age: E13.5 tissue: palatal shelf cell type: mouse embryonic palate (MEP) primary cells treatment: induction with pre-clustered ephrin-B1
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Treatment protocol |
Forward signaling was induced by treatment with 2 ug/ml of ephrin-B1-Fc pre-clustered with an anti-human antibody.
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Growth protocol |
MEP cells were isolated and grown in DMEM + 10%FBS, and passaged to P1 before induction of signaling.
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Extracted molecule |
total RNA |
Extraction protocol |
QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
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Hybridization protocol |
Standard Illumina hybridization protocol.
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Scan protocol |
Standard Illumina scanning protocol.
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Description |
1905531021_E Induced with 2 ug/ml pre-clustered ephrin-B1-Fc for 60 minutes.
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Data processing |
Data was normalized using cubic spline in Illumina Genome Studio v1.6.0 software; no background subtraction was used.
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Submission date |
Aug 10, 2010 |
Last update date |
Sep 21, 2010 |
Contact name |
Jeffrey Ohmann Bush |
E-mail(s) |
jeffrey.bush@mssm.edu, johmannbush@gmail.com
|
Organization name |
Mount Sinai School of Medicine
|
Department |
Developmental and Regenerative Biology
|
Lab |
Soriano
|
Street address |
One Gustave Levy Place, Box 1020
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10029 |
Country |
USA |
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|
Platform ID |
GPL6105 |
Series (1) |
GSE23539 |
Identification of immediate early transcriptional targets of ephrin-B1 forward signaling |
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