|
| Status |
Public on Sep 21, 2010 |
| Title |
MEP_induced_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
MEP cells isolated from E13.5 palatal shelves, induced
|
| Organism |
Mus musculus |
| Characteristics |
strain: CBA;C57BL6 N2
developmental stage: embryo
age: E13.5
tissue: palatal shelf
cell type: mouse embryonic palate (MEP) primary cells
treatment: induction with pre-clustered ephrin-B1
|
| Treatment protocol |
Forward signaling was induced by treatment with 2 ug/ml of ephrin-B1-Fc pre-clustered with an anti-human antibody.
|
| Growth protocol |
MEP cells were isolated and grown in DMEM + 10%FBS, and passaged to P1 before induction of signaling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
4032131007_E
Induced with 2 ug/ml pre-clustered ephrin-B1-Fc for 60 minutes.
|
| Data processing |
Data was normalized using cubic spline in Illumina Genome Studio v1.6.0 software; no background subtraction was used.
|
| |
|
| Submission date |
Aug 10, 2010 |
| Last update date |
Sep 21, 2010 |
| Contact name |
Jeffrey Ohmann Bush |
| E-mail(s) |
jeffrey.bush@mssm.edu, johmannbush@gmail.com
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Developmental and Regenerative Biology
|
| Lab |
Soriano
|
| Street address |
One Gustave Levy Place, Box 1020
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE23539 |
Identification of immediate early transcriptional targets of ephrin-B1 forward signaling |
|
