|
| Status |
Public on Oct 27, 2010 |
| Title |
Monocyte-derived Dc #1 |
| Sample type |
RNA |
| |
|
| Source name |
in vitro differentiated Monocyte-derived Dendritic cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood (buffy coat)
cell type: Monocyte-derived dendritic cell
|
| Growth protocol |
monocyte derived DC: CD14+ monocyte were cultured in the presence of IL4 and GMCSF, other DC subtypes were not treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit was used to extract total RNA (according to the manufacturer's instructions).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using 3` IVT Expression kit (Affymetrix).
|
| |
|
| Hybridization protocol |
10 ug of fragmented biotinylated cRNA was hybridized for 16 hr at 45C on GeneChip HG133_plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
A88
|
| Data processing |
CHP file were generated by Affymetrix Expression Console, using RMA algorithm
|
| |
|
| Submission date |
Aug 13, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
LAJOS SZELES |
| E-mail(s) |
szelesl@dote.hu
|
| Organization name |
UNIVERSITY OF DEBRECEN
|
| Street address |
Egyetem tér
|
| City |
DEBRECEN |
| ZIP/Postal code |
H-4028 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23618 |
Transcriptome profiling of dendritic cell subtypes |
|
| Relations |
| Reanalyzed by |
GSE86362 |
