|
| Status |
Public on Aug 15, 2010 |
| Title |
RCC parental cell line (non-metastatic), biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SN12C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Qiagen miRNeasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Scanner GSC3000 M10 type.
|
| Description |
Expression data from RCC cell lines to study metastasis progression
|
| Data processing |
The data were analyzed with Partek Genomic Suite 6.5 using default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Aug 13, 2010 |
| Last update date |
Aug 13, 2010 |
| Contact name |
Venkata J Thodima |
| E-mail(s) |
tvjagan@gmail.com
|
| Phone |
2126393595
|
| Fax |
2127173541
|
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Department |
Cell Biology
|
| Lab |
Chaganti
|
| Street address |
430 E 67th St RRL#337
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE23627 |
Expression data from RCC cell lines to study metastasis progression |
| GSE23631 |
RCC cell lines and paired tumors |
|
