|
| Status |
Public on Jan 20, 2011 |
| Title |
S.LEW(10)x12x2x3x5 _2 |
| Sample type |
RNA |
| |
|
| Source name |
S.LEW(10)x12x2x3x5 Congenic Rat fed with Low-salt (0.3%) diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: S.LEW(10)x12x2x3x5 Congenic Rat Heart
genotype: Congenic rat with Dahl S background
age: 53 days old S.LEW(10)x12x2x3x5 Congenic Rat
|
| Treatment protocol |
After weaning all rats were fed with Low-salt (0.3%) diet (Harlan Teklad diet TD 7034; Harlan–Sprague-Dawley).
|
| Growth protocol |
All rats born on the same day were selected and weaned at 30 days of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
S.LEW(10)x12x2x3x5 _2
|
| Data processing |
We have used R statistical program with the affylmGUI (Affymetrix .CEL files) to do the evaluation of data set. The data was normalized using Robust Multiarray Averaging (RMA). After normalization, Linear Model Fit was completed. Contrast parameters - Experiment versus control were used to create the 'Table of Genes Ranked in order of Differential Expression'. The p values were adjusted using the Benjamini-Hochberg method (BH).
|
| |
|
| Submission date |
Aug 16, 2010 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Bina Joe |
| E-mail(s) |
bina.joe@utoledo.edu
|
| Phone |
419-383-4415
|
| Fax |
419-383-2871
|
| Organization name |
University of toledo
|
| Department |
Physiology and Pharmacology
|
| Lab |
Physiological Genomics Laboratory
|
| Street address |
3000 Arlington avenue
|
| City |
Toledo |
| State/province |
OH |
| ZIP/Postal code |
43614 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE23643 |
Cardiac transcriptome profiles of S.LEW congenic strain compared with the hypertensive Dahl S rat |
|
