|
| Status |
Public on Dec 31, 2013 |
| Title |
Vehicle-24h-Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Right cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: Male
weight: 250-300g
treatment: Vehicle
treatment time-point: 24h
|
| Treatment protocol |
Cerebral ischemia was induced by permanent occlusion of the left middle cerebral artery (MCA) using a subtemporal approach (Qu et al., 2006). Male Wistar rats (250 - 300 g) were anesthetized with ketamine (75mg/kg, intraperitoneal) and xylazine (10mg/kg, intraperitoneal). The MCA was exposed through a subtemporal craniectomy and cauterized from the point proximal to its origin to the point where it intersects the inferior cerebral vein. Sham group was operated in the same way as the experimental group without the occlusion of MCA.
|
| Growth protocol |
All procedures performed in this study were approved by the Institutional Animal Care and Use Committee of the National University of Singapore.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the conventional Trizol method and cleaned up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol with a hybridization duration of 17h.
|
| Scan protocol |
Standard Illumina scanning protocol with a pmt value=503 and scan factor=0.74.
|
| Description |
Replicate 3
5038770021_I
|
| Data processing |
The data were normalised using median normalisation with GeneSpring Ver 7.3
|
| |
|
| Submission date |
Aug 16, 2010 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
|
| Department |
Neuroscience group
|
| Lab |
A/P Steve Cheung
|
| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6101 |
| Series (2) |
| GSE23651 |
Global transcriptomic profiling of permanent focal ischemia in an in vivo rat model |
| GSE25676 |
Global gene regulation in permanent focal ischemia in an in vivo rat model |
|
