|
| Status |
Public on Mar 19, 2022 |
| Title |
A_3d_2 |
| Sample type |
SRA |
| |
|
| Source name |
Fungal hyphae
|
| Organism |
Orbilia oligospora |
| Characteristics |
time point: 3d
genotype: WT
|
| Treatment protocol |
Mycelium was collected and washed three times with 0.1 Mphosphate buffer saline (PBS) (pH 6.8-7.2).
|
| Growth protocol |
The WT, ΔAopex1 and ΔAopex6 mutant strains were cultured in PDA medium at 28°C for 5 d.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The WT, ΔAopex1 and ΔAopex6 mutant strains were cultured in PD shaking at 28 °C, 180 rpm for 3 and 5 days.
The libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
The data from high-throughput sequencing were analyzed using the OmicShare tools, a free online platform for data analysis (www.majorbio.com).
Genome_build: https://www.ncbi.nlm.nih.gov/assembly/GCF_000225545.1
Supplementary_files_format_and_content: Expression analysis table include TPM values for each sample
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Jan 19, 2022 |
| Last update date |
Mar 19, 2022 |
| Contact name |
Qianqian Liu |
| Phone |
17708850614
|
| Organization name |
Yunnan University
|
| Department |
School of Life Sciences
|
| Lab |
State Key Laboratory for Conservation and Utilization of Bio-Resources
|
| Street address |
No. 2, Cuihu North Road, Wuhua District, Kunming
|
| City |
Kunming |
| State/province |
Yunnan Province |
| ZIP/Postal code |
650091 |
| Country |
China |
| |
|
| Platform ID |
GPL31134 |
| Series (1) |
| GSE193953 |
Transcriptome analysis of AoPEX1 and AoPEX6 in nematode-trapping fungus Arthrobotrys oligospora |
|
| Relations |
| BioSample |
SAMN25087833 |
| SRA |
SRX13828875 |
