|
| Status |
Public on Mar 31, 2022 |
| Title |
genomic_DNA_from_Surgical_resection_GBM-23 |
| Sample type |
genomic |
| |
|
| Source name |
Surgical resection GBM-23
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 23
batch: 2
gender: Male
disease state: GBM
age: 42
treatment: TMZ+RT
os_month: 50.4328767123288
os_status: 0
study_origin: EORTC26981
tissue_type: FFPE
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using Qiagen AllPrep DNA/RNA Kit (Qiagen, 80204).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
grade IV glioma tumor sample from EORTC26981 study
|
| Data processing |
BeadStudio software v3.2 was used for primary data acqusition. Raw data was imported into R and further processed using R/Bioconductor package “minfi”. The raw pre-processing (Illumina method) was used for data normalization.
normalized (Illumina pre-processing) Average Beta
|
| |
|
| Submission date |
Jan 28, 2022 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Pierre Bady |
| E-mail(s) |
pierre.bady@unil.ch
|
| Phone |
0041 21 692 40 95
|
| Organization name |
CHUV / BCF / SIB
|
| Department |
Swiss Institute of Bioinformatics
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
Quartier UNIL-Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE195640 |
DNA methylation-based age acceleration observed in IDH wild-type glioblastoma is associated with better outcome - including in elderly patients |
|
