|
| Status |
Public on Aug 01, 2011 |
| Title |
980056N |
| Sample type |
RNA |
| |
|
| Source name |
980056N
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: gastric normal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from primary tissues and cell lines using the miRVana miRNA Isolation Kit (Ambion, Inc, Austin,TX,USA) according to manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Hybridization protocol |
RNA samples were hybridized to Agilent Human miRNA Microarrays (V2) representing 723 human and 76 human viral miRNAs.
|
| Scan protocol |
scanned with the Agilent DNA Microarray Scanner (Model G2565BA) miRNA expression values were normalized against background signals using Feature Extraction Software (Agilent).
|
| Description |
n/a
|
| Data processing |
The miRNA data was also subjected to a log 10 transformation followed by median centering across probes, prior to actual analysis.
|
| |
|
| Submission date |
Aug 20, 2010 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Chia-Huey Ooi |
| Organization name |
F. Hoffmann-La Roche Ltd.
|
| Street address |
Grenzacherstrasse 124
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE23739 |
miRNA expressions in non-cancerous and cancerous human gastric tissues |
|
