|
Status |
Public on Aug 01, 2011 |
Title |
980305N |
Sample type |
RNA |
|
|
Source name |
980305N
|
Organism |
Homo sapiens |
Characteristics |
tissue: gastric normal tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from primary tissues and cell lines using the miRVana miRNA Isolation Kit (Ambion, Inc, Austin,TX,USA) according to manufacturer’s instructions.
|
Label |
Cy3
|
Label protocol |
standard Agilent protocol
|
|
|
Hybridization protocol |
RNA samples were hybridized to Agilent Human miRNA Microarrays (V2) representing 723 human and 76 human viral miRNAs.
|
Scan protocol |
scanned with the Agilent DNA Microarray Scanner (Model G2565BA) miRNA expression values were normalized against background signals using Feature Extraction Software (Agilent).
|
Description |
n/a
|
Data processing |
The miRNA data was also subjected to a log 10 transformation followed by median centering across probes, prior to actual analysis.
|
|
|
Submission date |
Aug 20, 2010 |
Last update date |
Aug 01, 2011 |
Contact name |
Chia-Huey Ooi |
Organization name |
F. Hoffmann-La Roche Ltd.
|
Street address |
Grenzacherstrasse 124
|
City |
Basel |
ZIP/Postal code |
4070 |
Country |
Switzerland |
|
|
Platform ID |
GPL7731 |
Series (1) |
GSE23739 |
miRNA expressions in non-cancerous and cancerous human gastric tissues |
|