|
| Status |
Public on Jan 25, 2024 |
| Title |
LAN757A17 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Homo sapiens |
| Characteristics |
developmental stage: 14 pcw
Sex: male
number of chromosomes: 46 chromosomes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
samples were obtained and snap frozen and kept at -80C. Samples were lysed (buffer RLT Qiagen Rneasy Maxi kit) and homogenised using Macs Milteni M tubes and gentleMACS dissociator followed by RNA extraction using Qiagen Rneasy Maxi kit following the standard protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
control
|
| Data processing |
Read trimming and adaptor removal with Trimmomatic (v 0.36)
Read alignment with STAR ( v 2.5.2a) and gene quantification with RSEM( v 1.2.31)
Differential expression analysis DESeq2
Genome_build: GRCh38 Ensembl release 89
Supplementary_files_format_and_content: table of normalized counts per gene per sample obtained from DESeq
|
| |
|
| Submission date |
Feb 09, 2022 |
| Last update date |
Jan 25, 2024 |
| Contact name |
Victor Tybulewicz |
| E-mail(s) |
Victor.T@crick.ac.uk
|
| Organization name |
Francis Crick Institute
|
| Street address |
1 Midland Road
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20301 |
| Series (2) |
| GSE196443 |
Transcriptomic analysis of human foetal hearts with or without Down Syndrome |
| GSE196447 |
Down Syndrome |
|
| Relations |
| BioSample |
SAMN25816055 |
| SRA |
SRX14121132 |
