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| Status |
Public on May 15, 2024 |
| Title |
FourWT0m [WT - 1] |
| Sample type |
SRA |
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| Source name |
Yeast cell
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| Organism |
Schizosaccharomyces pombe |
| Characteristics |
strain: WT972
genotype: wild type
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| Growth protocol |
Cells were grown to log phase using YEA media at 30°C.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Asynchronous growing log phase cells were collected and lysed using glassbeads and FastPrep-24 homogenizer (MP Biomedicals, Santa Ana, CA). Total RNA was extracted using TRIzol (Thermo Fisher Scientific, Waltham, MA), followed by twice extraction with phenol:chloroform:isoamyl alcohol (25:24:1) and once with chloroform. Samples were subsequently treated with DNase I to eliminate the DNA. RNA-seq was performed by BGI (Shenzhen, China) using an Illumina HiSeqTM 2000 platform (Illumina, San Diego, CA).
cDNA
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Reads were mapped to S.pombe genome by STAR(version STAR_2.5.4b) with default parameters
featureCounts(Version 1.5.0-p1) generated gene expression
Genome_build: ASM294v2
Supplementary_files_format_and_content: tab-delimited text files include normalized gene expression for each sample.
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| Submission date |
Feb 10, 2022 |
| Last update date |
May 15, 2024 |
| Contact name |
Ying Li |
| E-mail(s) |
csiliyi@nus.edu.sg
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| Organization name |
Cancer Science Institute of Singapore
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| Street address |
14 Medical Dr, Singapore
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| City |
Singapore |
| State/province |
--- Select a state --- |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
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| Platform ID |
GPL13988 |
| Series (1) |
| GSE196504 |
Quantitative analysis of wild type and set2 mutant transcriptomes |
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| Relations |
| BioSample |
SAMN25844387 |
| SRA |
SRX14129804 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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