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Sample GSM5899229 Query DataSets for GSM5899229
Status Public on Feb 21, 2022
Title aspergillus_WT_2
Sample type SRA
 
Source name Aspergillus flavus NRRL3357
Organism Aspergillus flavus
Characteristics treatment: incubate for 3 days on GMM medium
genotype: wild type
strain: aspergillus flavus NRRL3357
Treatment protocol Add to the glucose minimal medium(spores of WT, ΔrgsA and ΔrgsE).
Growth protocol keep these strains at 29◦C for 48h and simultaneously need to be shook at 180rmp.
Extracted molecule total RNA
Extraction protocol The harvested-mycelia (WT, ΔrgsA and ΔrgsE) were frozen in liquid nitrogen, and were lyophilized for 1day. The RNA of WT, ΔrgsA and ΔrgsE strains was extracted by TRIzol reagent according to the manufacturer’s instruction.
RNA libraries were prepared for sequencing using standard Illumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Illumina Casava1.7 software used for basecalling
Raw sequencing data was first filtered by Trimmomatic (version 0.36), then mapped to the reference genome of aspergillus flavus using STRA software (version 2.5.3a).
Reads mapped to the exon regions of each gene were counted by featureCounts (Subread-1.5.1; Bioconductor) and then RPKMs were calculated. Genes differentially expressed between groups were identified using the edgeR package (version 3.12.1).
Genome_build: https://fungidb.org/fungidb/app/downloads/Current_Release/AflavusNRRL3357/
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...(
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
 
Submission date Feb 14, 2022
Last update date Feb 22, 2022
Contact name rui xie
E-mail(s) xr1083928195@outlook.com
Phone 18705003986
Organization name Fujian Agriculture and Forestry University
Street address shangxiadian street
City Fuzhou
ZIP/Postal code 350000
Country China
 
Platform ID GPL30388
Series (1)
GSE196717 Regulator of G protein signaling contributes to the development and aflatoxin biosynthesis in Aspergillus flavus through the regulation of Gα activity.
Relations
BioSample SAMN25943193
SRA SRX14173701

Supplementary file Size Download File type/resource
GSM5899229_WT2.txt.gz 117.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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