Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).
Label
Biotin
Label protocol
Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 200ng of total RNA. Incubation of labeling reaction: 14h.
Hybridization protocol
1.50 ug each sample was hybridized to Illumina’s SentrixÒ Mouse-6 Expression BeadChips (cat no BD-26-101) at 55 °C overnight (19 h) according to Illumina BeadStation 500X –protocol, Revision F.
Scan protocol
Chips were scanned with Illumina BeadArray Reader and the numerical results were extracted with Bead Studio v1.5.1.34
Data processing
Bead Studio v1.5.1.34
Submission date
Sep 09, 2010
Last update date
Dec 31, 2011
Contact name
Pei-wen Pan
Organization name
Institute of Medical Technology, University of Tampere
