|
| Status |
Public on Sep 30, 2023 |
| Title |
3.3yo Male High hyperdiploid Acute Lymphoblastic Leukemia Patient_12 |
| Sample type |
genomic |
| |
|
| Source name |
WBC from Bone Marrow
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: Patient_12
disease: B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL)
age: 3.3yo
gender: Male
|
| Treatment protocol |
treatment is according to IciCLe protocol of InPOG
|
| Growth protocol |
DNA extracted from mononuclear cell from Bone Marrow in EB at -20C
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from bone marrow at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, Qubit and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
hybridized to Affymetrix CytoScan HD
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software (GCOS version 1.4). Copy number values for individual SNPs were extracted and converted from CEL files into signal intensities using ChAS v1.5.
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Mar 03, 2022 |
| Last update date |
Sep 30, 2023 |
| Contact name |
Mayur Parihar |
| E-mail(s) |
mayur.parihar@ttcrc.tmckolkata.org
|
| Organization name |
Tata Medical Center
|
| Department |
Pathology
|
| Lab |
Cytogenetics and Genomics
|
| Street address |
14 Major Arterial Road (E-W)
|
| City |
Kolkata |
| State/province |
West Bengal |
| ZIP/Postal code |
700160 |
| Country |
India |
| |
|
| Platform ID |
GPL16131 |
| Series (1) |
| GSE197838 |
Affymetrix SNP array data for acute lymphoblastic leukemia samples |
|
