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Sample GSM593136 Query DataSets for GSM593136
Status Public on Sep 14, 2010
Title Sqadipose_t1_Adlib_a5
Sample type RNA
 
Source name Subcutaneous adipose tissue at baseline
Organism Canis lupus familiaris
Characteristics tissue: subcutaneous adipose
animal id: 5
time point: 0 weeks
treatment: Ad libitum
batch: 3
breed: Beagle
Treatment protocol Dogs were randomized to either ad libitum (n=5) or body weight maintenance (n=4) feeding. All dogs were fed Purina® Pro Plan® Performance Formula. All subcutaneous adipose samples were collected after an overnight fasting period.
Growth protocol Dogs were fed ad libitum (n=5) or to maintain baseline body weight (control; n=4).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol as suggested by the manufacturer.
Label biotin
Label protocol Biotin-labeled cRNA samples were made using Affymetrix GeneChip Expression 3'-Amplification Reagents (One-Cycle Target Labeling and Control Reagents package) according to the manufacturer's instructions.
 
Hybridization protocol Purified, fragmented cRNA was hybridized to Affymetrix's Canine 2.0 Genome Array using Affymetrix's GeneChip Fluidics Station 450, as per Affymetrix's instructions.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
Description Ad libitum fed dog gene expression data from subcutaneous adipose tissure at baseline
Data processing Pre-processing was done using the GCRMA algorithm in R and Bioconductor packages.
 
Submission date Sep 13, 2010
Last update date Sep 13, 2010
Contact name Kelly Scott Swanson
E-mail(s) ksswanso@uiuc.edu
Organization name University of Illinois
Street address 1207 W. Gregory Dr.
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platform ID GPL3738
Series (1)
GSE24099 Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression

Data table header descriptions
ID_REF
VALUE GCRMA log2 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 9.282898666
AFFX-BioB-5_at 9.265428135
AFFX-BioB-M_at 10.1700867
AFFX-BioC-3_at 11.58074239
AFFX-BioC-5_at 10.56182938
AFFX-BioDn-3_at 13.0403168
AFFX-BioDn-5_at 12.76931274
AFFX-Cf_Actin_3_at 14.38262251
AFFX-Cf_Actin_5_s_at 15.32023366
AFFX-Cf_Actin_M_at 12.17413973
AFFX-Cf_AdrenRecpt_3_at 2.444700085
AFFX-Cf_AdrenRecpt_5_at 1.857455757
AFFX-Cf_AdrenRecpt_M_at 3.152303999
AFFX-Cf_G-6-PDH_3_at 8.901890277
AFFX-Cf_G-6-PDH_5_at 2.618004477
AFFX-Cf_G-6-PDH_M_at 4.090945924
AFFX-Cf_Gapdh_3_at 15.77288192
AFFX-Cf_Gapdh_5_at 15.43482971
AFFX-Cf_Gapdh_M_at 14.07495497
AFFX-Cf_P450-2E1_3_s_at 2.471193673

Total number of rows: 43035

Table truncated, full table size 1356 Kbytes.




Supplementary file Size Download File type/resource
GSM593136_2472_E_5_Canine_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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