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Sample GSM593137 Query DataSets for GSM593137
Status Public on Sep 14, 2010
Title Sqadipose_t1_Adlib_a8
Sample type RNA
 
Source name Subcutaneous adipose tissue at baseline
Organism Canis lupus familiaris
Characteristics tissue: subcutaneous adipose
animal id: 8
time point: 0 weeks
treatment: Ad libitum
batch: 4
breed: Beagle
Treatment protocol Dogs were randomized to either ad libitum (n=5) or body weight maintenance (n=4) feeding. All dogs were fed Purina® Pro Plan® Performance Formula. All subcutaneous adipose samples were collected after an overnight fasting period.
Growth protocol Dogs were fed ad libitum (n=5) or to maintain baseline body weight (control; n=4).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol as suggested by the manufacturer.
Label biotin
Label protocol Biotin-labeled cRNA samples were made using Affymetrix GeneChip Expression 3'-Amplification Reagents (One-Cycle Target Labeling and Control Reagents package) according to the manufacturer's instructions.
 
Hybridization protocol Purified, fragmented cRNA was hybridized to Affymetrix's Canine 2.0 Genome Array using Affymetrix's GeneChip Fluidics Station 450, as per Affymetrix's instructions.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
Description Ad libitum fed dog gene expression data from subcutaneous adipose tissure at baseline
Data processing Pre-processing was done using the GCRMA algorithm in R and Bioconductor packages.
 
Submission date Sep 13, 2010
Last update date Sep 13, 2010
Contact name Kelly Scott Swanson
E-mail(s) ksswanso@uiuc.edu
Organization name University of Illinois
Street address 1207 W. Gregory Dr.
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platform ID GPL3738
Series (1)
GSE24099 Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression

Data table header descriptions
ID_REF
VALUE GCRMA log2 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 9.173199937
AFFX-BioB-5_at 9.095768422
AFFX-BioB-M_at 10.13890994
AFFX-BioC-3_at 11.30042733
AFFX-BioC-5_at 10.39579054
AFFX-BioDn-3_at 12.77486891
AFFX-BioDn-5_at 12.47589892
AFFX-Cf_Actin_3_at 14.48125084
AFFX-Cf_Actin_5_s_at 14.66049102
AFFX-Cf_Actin_M_at 12.29798543
AFFX-Cf_AdrenRecpt_3_at 2.799058456
AFFX-Cf_AdrenRecpt_5_at 1.888450122
AFFX-Cf_AdrenRecpt_M_at 3.191774757
AFFX-Cf_G-6-PDH_3_at 6.251184252
AFFX-Cf_G-6-PDH_5_at 1.991329201
AFFX-Cf_G-6-PDH_M_at 2.417372381
AFFX-Cf_Gapdh_3_at 15.41410199
AFFX-Cf_Gapdh_5_at 15.15862207
AFFX-Cf_Gapdh_M_at 13.67884541
AFFX-Cf_P450-2E1_3_s_at 2.629260466

Total number of rows: 43035

Table truncated, full table size 1356 Kbytes.




Supplementary file Size Download File type/resource
GSM593137_2477_E_8_Canine_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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