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Status |
Public on Sep 14, 2010 |
Title |
Sqadipose_t1_Adlib_a8 |
Sample type |
RNA |
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Source name |
Subcutaneous adipose tissue at baseline
|
Organism |
Canis lupus familiaris |
Characteristics |
tissue: subcutaneous adipose animal id: 8 time point: 0 weeks treatment: Ad libitum batch: 4 breed: Beagle
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Treatment protocol |
Dogs were randomized to either ad libitum (n=5) or body weight maintenance (n=4) feeding. All dogs were fed Purina® Pro Plan® Performance Formula. All subcutaneous adipose samples were collected after an overnight fasting period.
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Growth protocol |
Dogs were fed ad libitum (n=5) or to maintain baseline body weight (control; n=4).
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol as suggested by the manufacturer.
|
Label |
biotin
|
Label protocol |
Biotin-labeled cRNA samples were made using Affymetrix GeneChip Expression 3'-Amplification Reagents (One-Cycle Target Labeling and Control Reagents package) according to the manufacturer's instructions.
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Hybridization protocol |
Purified, fragmented cRNA was hybridized to Affymetrix's Canine 2.0 Genome Array using Affymetrix's GeneChip Fluidics Station 450, as per Affymetrix's instructions.
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Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
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Description |
Ad libitum fed dog gene expression data from subcutaneous adipose tissure at baseline
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Data processing |
Pre-processing was done using the GCRMA algorithm in R and Bioconductor packages.
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|
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Submission date |
Sep 13, 2010 |
Last update date |
Sep 13, 2010 |
Contact name |
Kelly Scott Swanson |
E-mail(s) |
ksswanso@uiuc.edu
|
Organization name |
University of Illinois
|
Street address |
1207 W. Gregory Dr.
|
City |
Urbana |
State/province |
IL |
ZIP/Postal code |
61801 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE24099 |
Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression |
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