|
| Status |
Public on Mar 05, 2022 |
| Title |
Mediastinal lymph node Patient 11 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor-free mediastinal lymph node
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tumor-free mediastinal lymph node
overall survival: 30
censored overall survival: No
progression-free survival: 9
censored progression-free survival: No
age at diagnosis in years: 65
Sex: Female
stage at diagnosis: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10 um-thick FFPE sections were prepared for the extraction of total RNA from tumor cell-rich areas, affected lymph nodes and medistinal and hilar lymph nodes. Areas of interest were identified by a expert pathologist using H&E staings and overlaying with cut FFPE sections. Extracion was performed using Qiagen FFPE RNEasy kits and mRNA concentration was measured via Nanodrop.
|
| Label |
33P
|
| Label protocol |
Labelling, hybridization and scanning was performed on the nCounter platform according to manufacturer's instructions (Nanostring Technologies nCounter Analysis System, Seattle, USA). Gene expression profiling was performed using the IO 360 panel.
|
| |
|
| Hybridization protocol |
Labelling, hybridization and scanning was performed on the nCounter platform according to manufacturer's instructions (Nanostring Technologies nCounter Analysis System, Seattle, USA). Gene expression profiling was performed using the IO 360 panel.
|
| Scan protocol |
Labelling, hybridization and scanning was performed on the nCounter platform according to manufacturer's instructions (Nanostring Technologies nCounter Analysis System, Seattle, USA). Gene expression profiling was performed using the IO 360 panel.
|
| Description |
Samples are 4 matched tissue from the same patients with 16 total patients
LK163-3
|
| Data processing |
Raw gene expression data was analyzed via Nanostring's nSolver 4.0 software. Quality control steps as well as normalization of data was performed.
|
| |
|
| Submission date |
Mar 04, 2022 |
| Last update date |
Mar 05, 2022 |
| Contact name |
Laura Sellmer |
| Organization name |
Department of Medicine V
|
| Street address |
Ziemssenstrasse 1
|
| City |
München |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL27956 |
| Series (1) |
| GSE197929 |
Gene expression based profiling of tumor and mediastinal and hilar lymph nodes in NSCLC |
|
