|
| Status |
Public on Mar 10, 2022 |
| Title |
D_sham1 |
| Sample type |
SRA |
| |
|
| Source name |
DRG Tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: SD
tissue: dorsal root ganglion (DRG)
treatment: sham
|
| Treatment protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| Growth protocol |
DRG tissue were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We extracted total RNA from approximately 150 mg of DRG tissue using a MirVana total RNA extraction kit (Ambion, Carlsbad, CA, United States). RNA levels were measured using the Qubit® RNA Assay Kit and a Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, United States). We assessed RNA integrity using an RNA Nano 6,000 Assay Kit of the Bioanalyzer 2,100 system (Agilent Technologies, CA, United States).
We used 1.5 μg of RNA per sample as the follow-up test material for RNA sample preparation. Whole transcriptome profiling was performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, United States) following the manufacturer’s instructions(Xu et al., 2021).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Description |
The skin was cut to expose the sciatic nerve and its three terminal branches directly through the part formed by the biceps muscle: the lateral side, common fibular nerve, and tibial nerves. Close the skin
|
| Data processing |
SNI transcriptomic data were obtained from Institute BGI data portal (https://biosys.bgi.com/) and the detailed sample numbers are listed in Supporting Information Tables S1. Student’s t‐test was also used for comparing the difference between SNI tissues and Sham tissues (P<0.05; |Fold change| > 2).
|
| |
|
| Submission date |
Mar 07, 2022 |
| Last update date |
Mar 10, 2022 |
| Contact name |
Zhen Zhen Xu |
| E-mail(s) |
d202081663@hust.edu.cn
|
| Phone |
+86-18771566212
|
| Organization name |
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Department |
Department of Anesthesiology
|
| Street address |
1277 Jiefang Avenue, Kong Hon District
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL32027 |
| Series (1) |
| GSE198020 |
Metabolomic and transcriptomic profiling of DRG in SNI rat reveal a common pattern of changes in metabolites and signaling pathways in Neuropathic Pain |
|
| Relations |
| BioSample |
SAMN26502266 |
| SRA |
SRX14394340 |
