|
| Status |
Public on Mar 20, 2022 |
| Title |
BL7_CR2W2 |
| Sample type |
SRA |
| |
|
| Source name |
Leucocytes
|
| Organism |
Bos taurus |
| Characteristics |
tissue: Leucocytes
diet: High grain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from blood leucocytes was extracted using NucleoSpin miRNA kit (Macherey-Nagel, Germany), while plasma total RNA was extracted with the NucleoSpin Plasma miRNA kit (Macherey-Nagel, Germany).
Library preparation was prepared using the NETflex Small RNA-Seq Kit (Bioo Scientific).
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Demultiplexing of the sequencing reads was performed with Illumina bcl2fastq (2.20).
Adapters were trimmed with Skewer (version 0.2.2) (Jiang et al. 2014).
Demultiplexed reads were analyzed using sRNAbench. Reads with an average PHRED score below 20 were discarded.
Assembly: UMD3_1_mp
Supplementary files format and content: tab-delimited text file include counts of all samples (sRNAbench output)
Supplementary files format and content: tab-delimited text file include DESeq2-normalized counts of all samples
|
| |
|
| Submission date |
Mar 17, 2022 |
| Last update date |
Mar 20, 2022 |
| Contact name |
Cátia Pacífico |
| E-mail(s) |
catia.s.pacifico@gmail.com
|
| Organization name |
University of Veterinary Medicine Vienna
|
| Lab |
Institute of Animal Nutrition and Functional Plant Compounds
|
| Street address |
Veterinaerplatz 1
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
1210 |
| Country |
Austria |
| |
|
| Platform ID |
GPL26012 |
| Series (1) |
| GSE198854 |
Circulating miRNAome of dry cows transitioned from forage to 65% grain diet |
|
| Relations |
| BioSample |
SAMN26749575 |
| SRA |
SRX14480835 |
