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Sample GSM5958016 Query DataSets for GSM5958016
Status Public on Mar 20, 2022
Title BL7_CR2W2
Sample type SRA
 
Source name Leucocytes
Organism Bos taurus
Characteristics tissue: Leucocytes
diet: High grain
Extracted molecule total RNA
Extraction protocol Total RNA from blood leucocytes was extracted using NucleoSpin miRNA kit (Macherey-Nagel, Germany), while plasma total RNA was extracted with the NucleoSpin Plasma miRNA kit (Macherey-Nagel, Germany).
Library preparation was prepared using the NETflex Small RNA-Seq Kit (Bioo Scientific).
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina NovaSeq 6000
 
Data processing Demultiplexing of the sequencing reads was performed with Illumina bcl2fastq (2.20).
Adapters were trimmed with Skewer (version 0.2.2) (Jiang et al. 2014).
Demultiplexed reads were analyzed using sRNAbench. Reads with an average PHRED score below 20 were discarded.
Assembly: UMD3_1_mp
Supplementary files format and content: tab-delimited text file include counts of all samples (sRNAbench output)
Supplementary files format and content: tab-delimited text file include DESeq2-normalized counts of all samples
 
Submission date Mar 17, 2022
Last update date Mar 20, 2022
Contact name Cátia Pacífico
E-mail(s) catia.s.pacifico@gmail.com
Organization name University of Veterinary Medicine Vienna
Lab Institute of Animal Nutrition and Functional Plant Compounds
Street address Veterinaerplatz 1
City Vienna
State/province Vienna
ZIP/Postal code 1210
Country Austria
 
Platform ID GPL26012
Series (1)
GSE198854 Circulating miRNAome of dry cows transitioned from forage to 65% grain diet
Relations
BioSample SAMN26749575
SRA SRX14480835

Supplementary file Size Download File type/resource
GSM5958016_S2502Nr49.txt.gz 30.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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