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| Status |
Public on Mar 21, 2022 |
| Title |
Peripheral blood [A584_019 card A ] |
| Sample type |
RNA |
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| Source name |
Peripheral blood
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| Organism |
Homo sapiens |
| Characteristics |
age (years): 83.0
tag: Postmenopausal women
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| Treatment protocol |
Not applicable
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| Growth protocol |
Not applicable
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from whole blood was isolated according to the PAXgene Blood RNA Kit manufacturer (BD, Franklin Lakes, NJ, USA), including the optional on-column DNase digestion according to manufacturer’s instructions.
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| Label |
FAM
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| Label protocol |
Total RNA (45 ng) was reverse-transcribed using Megaplex TM reverse transcription (RT) primersets A and B (Applied Biosystems, Foster City, CA, USA). TaqMan miRNA LDA Arrays A and B (Applied Biosystems) were used for profiling 758 different ncRNAs.
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
A584_019
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| Data processing |
The data were analyzed using SDS 2.3 software (Applied Biosystems, Foster City, CA, USA) and the comparative threshold cycle (ΔΔCt) method.
We used a restricted mean of expressed miRNAs, and normalized between two plates with the same sample and performed a normalization across samples.
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| Submission date |
Mar 18, 2022 |
| Last update date |
Mar 21, 2022 |
| Contact name |
Sjur Reppe |
| E-mail(s) |
reppe.sjur@gmail.com
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| Phone |
+47 40614313
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| Organization name |
OSLO UNIVERSITY HOSPITAL
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| Department |
Medical biochemistry
|
| Lab |
Tisue culture
|
| Street address |
Kirkeveien 166
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| City |
Oslo |
| ZIP/Postal code |
0450 |
| Country |
Norway |
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| Platform ID |
GPL17039 |
| Series (2) |
| GSE198933 |
In silico discovery of the blood cell interactome [microRNA] |
| GSE198941 |
In silico discovery of the blood cell interactome |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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