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Sample GSM596114 Query DataSets for GSM596114
Status Public on Sep 21, 2010
Title Masseter muscle_AQM_Control, rep2
Sample type RNA
Source name masseter muscle, untreated
Organism Sus scrofa
Characteristics breed: Eurasian wild boar
gender: female
tissue: masseter muscle
disease: acute quadriplegic myopathy (AQM)
treatment: untreated
time: 1 day
Treatment protocol Five female domestic piglets (Sus scrofa, average body weight 26.9 kg) were used in this study. Piglets were immobilized by anesthesia and mechanically ventilated via a tracheotomy for a period of five days. During this study period, the animals were sedated using isoflurane inhalation (Abbott Laboratories, Chicago, Il, USA, 0.8 – 1.3% end-tidal concentration) supplemented by intravenous bolus doses of morphine and ketamine as needed. Core body temperature (blood) was maintained in the range of 38.5 – 40°C by a servo controlled heating pad. The animals received intravenous crystalloid fluid (Ringeracetat) to maintain stable blood pressure and urinary output and a glucose infusion (Rehydrex, Fresenius Kabi, Stockholm, Sweden, 25 mg glucose /mL) in the range of 0.5 – 1.5 mg/kg/minute to decrease the effects of catabolism. Each animal received prophylactic streptomycin 750 mg/d and bensylpenicillin 600 mg/d (Streptocillin Vet, Boeringer-Ingelheim, Hellerup, Denmark). Arterial blood gas analysis as well as electrolytes and blood glucose levels were monitored regularly and kept in the normal range throughout the study period. A neuromuscular blocking agent (NMBA) was administered as a continuous infusion of pancuronium bromide 0.1mg/kg/h (Pavolun; Organon, Boxtel, The Netherlands) for 5 days while a corticosteroid (CS) was given as bolus doses of betamethasone 0.1 mg/kg (Betapred; GSK, Slona, Sweden) twice daily for 5 days. Endotoxemia was induced by a continuous infusion of Escherichia coli endotoxin, serotype O26:B6 (Sigma Labkemi, Stockholm, Sweden) at 36 µg/kg/h for 1h.
Growth protocol All piglets originated from the same farm (Vallrums Lantbruk, Ransta, Sweden).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description PA079_P14D1_090128_(porcine).
Data processing The data were normalized using RMA.
Submission date Sep 20, 2010
Last update date May 03, 2011
Contact name sudhakar reddy aare
Organization name uppsala university
Department neuroscience
Lab clinical neurophysiology
Street address Ing 85, 3 tr, Akademiska sjukhuset
City uppsala
ZIP/Postal code 751 85
Country Sweden
Platform ID GPL3533
Series (1)
GSE24239 Mechanisms underlying the sparing of masticatory muscle function relative to biceps femoris muscle in an experimental critical illness model

Data table header descriptions
VALUE RMA-normalized log2 signal

Data table
Ssc.24411.3.S1_a_at 8.9370578863352
Ssc.30980.1.A1_at 8.93999830648167
Ssc.13711.1.S1_at 8.34637979549377
Ssc.30055.1.A1_at 6.82713753890697
Ssc.7331.1.S1_at 10.6938725416304
Ssc.27097.1.A1_at 11.6301840837856
Ssc.14528.1.S1_at 10.1048902306166
Ssc.20310.1.S1_at 8.99711107474146
Ssc.13266.3.A1_at 8.20940838715332
Ssc.4462.1.S1_at 11.0204278587276
Ssc.4231.1.A1_at 8.50935709860995
Ssc.4336.1.A1_at 6.44788777042673
Ssc.17974.1.A1_at 7.50788038945256
Ssc.11746.1.A1_at 5.82694088070111
Ssc.26153.1.A1_at 6.72863064610186
Ssc.24606.1.A1_a_at 8.15386837557364
Ssc.2886.1.S1_at 9.34095009252667
Ssc.30104.1.A1_at 9.66824054994295
Ssc.25077.1.S1_at 9.08344756277694
Ssc.1897.1.A1_at 7.77775971683305

Total number of rows: 24123

Table truncated, full table size 816 Kbytes.

Supplementary file Size Download File type/resource
GSM596114.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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