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Status
Public on Apr 02, 2022
Title
Study participant 100-136
Sample type
RNA
Source name
Primary p16(+) oropharyngeal squamous cell carcinoma
Organism
Homo sapiens
Characteristics
age(years): 45 sex (1=male,2=female): 1 race (1=white,2=asian): 1 tobacco (1=current,2=former,3=never): 3 subsite(1=tonsils and pillars,2=base of tongue,3=pharyngeal overlapping): 1 clin_t_stage(1=t0,2=t1,3=t2,4=t3,5=t4a,6=t4b): 3 clin_n_stage(1=n0,2=n1,3=n2,4=n3): 2 clin_m_stage(1=m0,2=m1): 1 clin_stage(1=stage i,2=stage ii,3=stage iii,4=stage iv): 1 recurrence(0=no recurrence,1=recurrence): 0 recurrence_time(days): 1811 vital_status(0=alive,1=dead): 0 os_time(days): 1811 disease state: human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) tissue: HPV(+) OPSCC tumor
Growth protocol
NA
Extracted molecule
total RNA
Extraction protocol
RNA was extracted from samples using QIAGEN RNeasy kits
Label
n/a
Label protocol
Hybridized transcripts were purified and immobilized on a sample cartridge
Hybridization protocol
Transcripts were hybridized to a panel of 770 probes (Nanostring IO360)
Scan protocol
Counts of hybridized transcripts were read using the nCounter platform
Data processing
Raw counts were normalized with the nSolver software (4.0) package using the geometric mean of both positive control probes and housekeeping probes. A background threshold count was set to 20.
Submission date
Mar 20, 2022
Last update date
Apr 02, 2022
Contact name
Jose P Zevallos
E-mail(s)
jpzevallos@wustl.edu
Organization name
Washington University School of Medicine
Department
Otolaryngology - Head and Neck Surgery
Street address
660 South Euclid Avenue, Campus Box 8115
City
St Louis
State/province
MO
ZIP/Postal code
63110
Country
USA
Platform ID
GPL32069
Series (1)
GSE199029
Integrative genome analysis reveals low T cell infiltration as the primary feature of tobacco use in HPV(+) oropharyngeal cancer
Data table header descriptions
ID_REF
VALUE
Normalized hybridization counts
Data table
ID_REF
VALUE
A2M
4894.56
ACVR1C
101.74
ADAM12
2337.66
ADGRE1
20
ADM
11739.18
ADORA2A
106.59
AKT1
4867.91
ALDOA
36454.21
ALDOC
719.47
ANGPT1
102.95
ANGPT2
234.98
ANGPTL4
718.26
ANLN
1761.12
APC
1001.68
APH1B
66.62
API5
4867.91
APLNR
363.37
APOE
6903.98
APOL6
3453.2
AQP9
129.6
Total number of rows: 760 Table truncated, full table size 9 Kbytes .Supplementary data files not provided
Processed data included within Sample table