|
Status |
Public on Sep 22, 2010 |
Title |
193231 DFI>300 |
Sample type |
RNA |
|
|
Source name |
Canine primary tumor from good responder cohort
|
Organism |
Canis lupus familiaris |
Characteristics |
tissue: snap frozen primary tumor genotype(breed): Mix age (years): 12.4
|
Growth protocol |
Primary tumor collected at time of limb amputation
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA followed by Qiagen Rneasy cleanup was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
One-Cycle Target Labeling and Control Reagents package (Affymetrix, Santa Clara, CA, USA) was used to synthesize cDNA from total RNA spiked with prokaryotic Poly-A RNA as a control. The Sample Cleanup Module (Affymetrix, Santa Clara, CA, USA) was used to purify the cDNA which was then used for synthesis of biotin-labeled cRNA.
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|
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Hybridization protocol |
cRNA was purified, quantified and fragmented before hybridization with the GeneChips. Hybridized chips were washed, stained using streptavidin-conjugated phycoerythrin dye (Invitrogen, Carlsbad, CA, USA) and enhanced with biotinylated goat anti-streptavidin antibody (Vector Laboratories, Burlingame, CA, USA) using an Affymetrix GeneChip Fluidics Station 450.
|
Scan protocol |
The Affymetrix GeneChip scanner 3000 was used to acquire images.
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Description |
Gene expression data from primary tumor
|
Data processing |
The data were analyzed with Expression Console (PLIER).
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|
|
Submission date |
Sep 21, 2010 |
Last update date |
Sep 21, 2010 |
Contact name |
Liza Emily O'Donoghue |
E-mail(s) |
lpfaff@rams.colostate.edu
|
Phone |
7739836434
|
Organization name |
Colorado State University
|
Department |
Clinical Sciences
|
Lab |
Duval
|
Street address |
300 W. Drake Rd
|
City |
Fort Collins |
State/province |
CO |
ZIP/Postal code |
80523 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE24251 |
Expression data from canine osteosarcoma primary tumors (Affymetrix Canine 2.0 array) |
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