|
| Status |
Public on Jun 10, 2011 |
| Title |
large airway [DGM-00529] |
| Sample type |
RNA |
| |
|
| Source name |
large airway
|
| Organism |
Homo sapiens |
| Characteristics |
department of genetic medicine id: DGM-00529
airway region: large airway
cell type: mixture of epithelium, basal, ciliated, secretory, undifferentiated and inflammatory cells
time: n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
DGM-00529_lg.CHP
DGM-00529_lg.CEL
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 24, 2010 |
| Last update date |
Jun 10, 2011 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (5)
|
| GSE24337 |
The Human Airway Epithelial Basal Cell Transcriptome |
| GSE32030 |
Empirical Bayes Conditional Independence Graphs for Dense Regulatory Network Recovery |
| GSE34450 |
Genes Associated with MUC5AC Expression in the Human Airway Epithelium |
| GSE77658 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes [array] |
| GSE77659 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes |
|
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