|
| Status |
Public on Oct 20, 2010 |
| Title |
Human ES cells replicate 1 - size Fraction 3 |
| Sample type |
SRA |
| |
|
| Source name |
human ES cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HES2
selected size: 2000-3500bp
|
| Treatment protocol |
Cells were treated with cycloheximide for 10 minutes prior to harvesting (Kolle et al., 2009)
|
| Growth protocol |
HES2 cells were cultured as per Kolle et al., Stem Cells 2009
|
| Extracted molecule |
total RNA |
| Extraction protocol |
SOLiD Whole transcriptome analysis Kit - RNA fragmented to ~100bp, RNA-DNA hybrid adaptors ligated, cDNA made, PCR amplified and gel purified
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB SOLiD System 2.0 |
| |
|
| Description |
mRNA from human ES cells selected for size (2000-3500bp)
|
| Data processing |
Raw data processed with Bioscope 1.2
|
| |
|
| Submission date |
Sep 24, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Sean Grimmond |
| E-mail(s) |
s.grimmond@imb.uq.edu.au
|
| Organization name |
University of Queensland
|
| Department |
Institute for Molecular Bioscience
|
| Lab |
Expression Genomics Lab
|
| Street address |
Services Rd
|
| City |
St Lucia |
| State/province |
Q |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9138 |
| Series (1) |
| GSE24355 |
Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human Embryonic Stem cells |
|
| Relations |
| SRA |
SRX029049 |
| BioSample |
SAMN00116345 |
