|
| Status |
Public on Oct 01, 2011 |
| Title |
IPC+IPO-32 |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
ischemia protocol: IPC + 30 min of total liver ischemia + IPO
|
| Treatment protocol |
Animals were anaesthetized using 0.75 ml/kg Hypnorm s.c. (fentanyl/fluanisone; Jansen Pharma, Birkeroed, Denmark) and 4 mg/kg Midazolam s.c. (dormicum; La Roche, Basel, Switzerland) and placed on a heated pad. A midline laparotomy was performed and the hepatoduodenal ligament was mobilised. Liver biopsies were sampled from the right lobe and immediately snap frozen in liquid nitrogen, and stored at -80°C until analysis.
|
| Growth protocol |
Male Wistar rats, weighing 300–350 g, (M&B Taconic, Eiby, Denmark) were housed in standard animal laboratories with the temperature maintained at 23°C and artificial 12-hour light-dark cycles, and with free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biopsies were homogenized in RLT plus buffer on Qiagen Tissuelyser. Total RNA was extracted on the QIAsymphony SP using the QIAsymphony RNA extraction kit.
|
| Label |
Biotin
|
| Label protocol |
150 ng of total RNA was used as target preparation in the starting material for the cDNA Syntesis Kit and WT Terminal Labeling Kit (affymetrix).
|
| |
|
| Hybridization protocol |
The target was loaded onto the GeneChip and hybridised for 16 h. The arrays were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using Affymetrix 7G GeneChip Scanner.
|
| Description |
IPC+IPO-32
|
| Data processing |
Raw image files from the quantitative scannings were analysed by Affymetrix Gene Expression Analysis Software and data were normalized using the RMA in Affymetrix Expression Console.
|
| |
|
| Submission date |
Sep 29, 2010 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Anders Riegels Knudsen |
| E-mail(s) |
auknudsen@gmail.com
|
| Organization name |
Aarhus University Hospital
|
| Department |
Department of Surgery, Section for upper GI surgery
|
| Street address |
Nørrebrogade 44, building 1c, LVN-research
|
| City |
Aarhus C |
| ZIP/Postal code |
8000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE24430 |
Ischemic Pre- and Post Conditioning has pronounced effects on Gene Expression Profiles in the Rat Liver after Ischemia/Reperfusion |
|
