|
| Status |
Public on Aug 15, 2023 |
| Title |
HN_WT4 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6/J
tissue: Heart
treatment: ex vivo treated
genotype: wild type
|
| Treatment protocol |
Heart slices were incubated under hypoxic conditions (3% O2) for one week followed by normoxic conditions (21% O2) for one week
|
| Growth protocol |
8 week old adult mice were sacrificed via cervical dislocation to harvest hearts and prepare heart slices
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIreagent according the manufactures protocol
TruSeq RNA Library Prep Kit v2
mRNA with Paired-End sequencing
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
treated_WT4
|
| Data processing |
50 million unique mapped reads were obtained from each RNA-seq experiment. The reads were aligned using STAR aligner.
Read count was calculated with GenomicFeatures Bioconductor package.
Calculation and normalization of “transcripts per kilobase million (TPM)” accounting for reads per kilobase (RPK) was performed as described elsewhere
Assembly: mm10
Supplementary files format and content: Data represented TPM values to ENSMBLIDs for each time point and replicate
|
| |
|
| Submission date |
Apr 07, 2022 |
| Last update date |
Aug 15, 2023 |
| Contact name |
Phillip Grote |
| E-mail(s) |
ph.grote@georg-speyer-haus.de
|
| Phone |
+49(69)63395620
|
| Organization name |
Georg-Speyer-Haus
|
| Department |
Genome Regulation
|
| Lab |
Grote
|
| Street address |
Paul-Ehrlich-Str. 42-44
|
| City |
Frankfurt am Main |
| State/province |
Hessen |
| ZIP/Postal code |
60596 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE200380 |
The lncRNA Sweetheart regulates adaptive cardiac hypertrophy after myocardial injury |
|
| Relations |
| BioSample |
SAMN27403010 |
| SRA |
SRX14775479 |
