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| Status |
Public on Apr 15, 2022 |
| Title |
Wnt+ adipocytes, without LF3 treatment, rep2 |
| Sample type |
SRA |
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| Source name |
immortalized stromal cells derived from iBAT
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| Organism |
Mus musculus |
| Characteristics |
tissue: immortalized stromal cells derived from iBAT
genotype: Tcf/Lef-H3B-gfp
treatment: adipogenic differentiation
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue or cells according to protocol for the RNeasy Lipid Tissue Mini Kit (Qiagen, 74804) or RNeasy Mini Kit (Qiagen, 27106) accordingly.
Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs) according to the manufacturer’s instruction.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 1000 |
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| Description |
ctrl-2
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| Data processing |
HISAT2, mapping
featureCounts, quantification
DESeq2, differential gene expression analysis
Assembly: mm10
Supplementary files format and content: DEGs
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| Submission date |
Apr 12, 2022 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Zhi Liu |
| E-mail(s) |
zliu23@tulane.edu
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| Phone |
5047566789
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| Organization name |
Tulane
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| Street address |
2734 CALHOUN
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| City |
NEW ORLEANS |
| State/province |
United States |
| ZIP/Postal code |
70118 |
| Country |
USA |
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| Platform ID |
GPL32159 |
| Series (1) |
| GSE164747 |
Discovery and functional assessment of a novel adipocyte population driven by intracellular Wnt/β-catenin signaling in mammals |
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| Relations |
| BioSample |
SAMN27534283 |
| SRA |
SRX14830695 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6040944_Ctrl_2_raw_counts.txt.gz |
122.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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